牛结蛋白基因启动子的初步改造研究

doi:10.11843/j.issn.0366-6964.2016.12.010

Abstract

Abstract:

On the basis of previous results, we transformed a bovine Desmin promoter fragment to obtain some high efficiency and muscle tissue-specific promoters which could be useful. We obtained 3 artificial Desmin promoter fragments successfully through adding several positive specific regulatory elements into the bovine Desmin promoter of 300 bp length, which included pGL3-desmin300-desmin180, pGL3-MD-αD①-desmin300 and pGL3-αD②-αD①-desmin300. The results showed that the activities of pGL3-desmin300-desmin180, pGL3-MD-αD①-desmin300 and pGL3-αD②-αD①-desmin300 were 1.67, 2.56 and 2.88 times more than activity of pGL3-desmin300, respectively. Bovine skeletal muscle satellite cells and fibroblast cell experiments showed that the 3 artificial Desmin promoter fragments had high muscle specificity. The application of Desmin fragments with high efficiency and muscle specificity will provide an important help to increase the output of muscle production of transgenic livestock.

CLC Number:

S823
S813.2

QI Li-si, TONG Hui-li, LI Shu-feng, HU Qian, YAN Yun-qin. The Study of Recombination of Bovine Desmin Gene Promoter[J]. ACTA VETERINARIA ET ZOOTECHNICA SINICA, 2016, 47(12): 2405-2413.

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The Study of Recombination of Bovine Desmin Gene Promoter

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